Coding

Part:BBa_K2220020:Design

Designed by: Nan Rong   Group: iGEM17_BNU-China   (2017-10-22)


Display beta-tubulin protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 325
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 641
    Illegal BamHI site found at 388
    Illegal BamHI site found at 1424
    Illegal XhoI site found at 1726
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1795
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1777
    Illegal BsaI.rc site found at 985
    Illegal SapI site found at 1521


Design Notes

We have done the codon optimization.


Source

Aga2 comes from pYD1 plasmid. Beta-tubulin: http://www.ncbi.nlm.nih.gov/nucleotide/645912969

References